The Ki-67+ cells were greatly increased after MSCs treatment. (CS), Intraperitoneal, Polymicrobial, and Antibiotics available under aCC-BY 4.0 International license. Mesenchymal Stem Cells Reduce Colitis in Mice via Release of TSG6, Independently of Their Localization to the Intestine. (A,B) Representative colon images and quantification of colon length; (C) myeloperoxidase (MPO) activity in colonic protein extracts; (D,E) Representative H&E staining and corresponding severity score; (F,G) Representative sirius red staining and quantification of collagen deposition. (A) Representative IHC images of each MSCs treatment group. Intraperitoneal injection is not always a suitable alternative to Srivastava, A. K. & Bulte, J. W. Seeing stem cells at work in vivo. If injecting a hazardous substance (biohazard, radiation hazard or chemical hazard), include precautions in the Animal Care Protocol for human safety (e.g. Methods of euthanasia likely to elicit distress vocalizations or pheromones that other animals could hear or smell should be performed in another location, if transportation distress can be minimized. ADS [6] In 1968, melatonin was delivered to rats intraperitoneally in order to study how brain serotonin would be affected in the midbrain. In a previous study, 14 mice were exposed to SWCNTs through intravenous (IV) injection to determine whether there were any adverse effects caused by SWCNTs on the mouse brain and also to find a safe application dosage if possible. This work is licensed under a Creative Commons Attribution 4.0 International License. Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy. Skin application of non-rodents (miniature pigs) study on peritoneal cells (PCs) migration to gut may give us some hints27. CAS Cytokine in colonic protein extracts were determined by ELISA Kits according to the manufacturers instructions (R&D Systems, Minneapolis, MN). It was concluded that there is evidence that IP may not be the best method of euthenasia due to possibilities of missinjection. Further study demonstrated TSG-6 alone was sufficient to reduce intestinal inflammation in mice with colitis. Intraperitoneal Injection. PDF EUTHANASIA REFERENCE MANUAL - HumanePro Cite this article. https://doi.org/10.1038/srep30696. The few examples that do exist pertain to the treatment of pancreatic/ovarian cancers and injections of other drugs in clinical trials. Thus there is a strong impetus to seek more effective approaches for disease management. Admittedly, there may be an overlook of MSCs existence due to the tissue selection of frozen section and sensitivity of fluorescence imaging, the therapeutic differentiation of MSCs in our study were not clearly observed. Appendix E: Common Vehicles for Nonclinical Evaluation of Therapeutic Comparative analysis of chemokine receptors expression in mesenchymal stem cells derived from human bone marrow and adipose tissue. Samples were frozen and thawed 3 times, centrifuged at 30,000g for 20minutes. [2] Intraperitoneal injections can be similar to oral administration in that hepatic metabolism could occur in both. Stem cells and development 18, 683692, doi: 10.1089/scd.2008.0253 (2009). Injection Techniques, Restraint, & Handling for Mice and Rats Distribution of GFP+ MSCs in colon 1 day after injection. was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity . Near-infrared imaging and quantification of MSCs transplants. Makhija, S. et al. To varying degrees, all the three MSCs delivery ways could ameliorate DSS-induced colitis. [8], A good example of how intraperitoneal injections work is depicted through "The distribution of salicylate in mouse tissues after intraperitoneal injection" because it includes information on how a drug can travel to the blood, liver, brain, kidney, heart, spleen, diaphragm, and skeletal muscle once it has been injected intraperitoneally.[9]. Muller-Ehmsen, J. While this route is an easy to master, quick, suitable for chronic treatments and with low impact of stress on laboratory rodents, there is a common concern that it may not be an acceptable route for drug administration in experimental studies. [20] There needs to be more research done to determine appropriate dosing and combinations of these drugs to advance intraperitoneal drug delivery. All the tissue sections in this study were scan and analyzed with Virtual slide scanning system (VS120-S, Olympus). During the observation, the body weight changes and fecal blood and their survival date was faithfully record. Article If there is negative pressure, proceed with the injection depress the plunger until the solution has been fully administered. Gastroenterology 114, 433440 (1998). adult mice at 8-10 weeks of age were first anaesthetized by intraperitoneal injection of 20 mg/ml Avertin (Sigma-Aldrich) (15 l/g . Neuronal cell death has been reported in retinas of humans with diabetic retinopathy and in diabetic rat models. The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion. Unauthorized use of these marks is strictly prohibited. Ko, I. K. et al. Because of the injection of cannabis, the patient was predicted to have some level of THC in the blood from absorption. Evid Based Complement Alternat Med. The following approaches are novel, and require further testing in humans before being accepted as potential treatment options: The medium was refreshed every 23 days and third- and fourth-passage cells were used for all experiments. Epub 2010 Mar 3. Our findings showed that the GFP+ MSCs were migrated to the inflamed colon, even passed through the whole intestinal wall and reached the luminal side. Bethesda, MD 20894, Web Policies Liu, T. et al. Human mesenchymal stem cells license adult CD34+ hemopoietic progenitor cells to differentiate into regulatory dendritic cells through activation of the Notch pathway. Two patients with long-standing ulcerative colitis, psoriasis and leukemia underwent allogeneic stem cell transplantation and all the three disorders were in clinical remission for 4 years after transplantation11. Cancers | Free Full-Text | Targeted Vaccination against Human Other gap junction blockers were also occasionally reported to . Rep. 6, 30696; doi: 10.1038/srep30696 (2016). Disease recovery was evaluated by histological server score, gross body weight and survival rate. Samples were centrifuged at 30,000g for 20minutes and stored at 80C until cytokine determination. . Horses and other large animals: 1 mL per 10 lb of body weight, to a maximum . Sphingosine-1-phosphate links persistent STAT3 activation, chronic intestinal inflammation and development of colitis-associated cancer. At 72h, the IP groups showed significant higher level of TSG-6 than AI groups and DSS control (P=0.023 and P=0.005, respectively). An example of when intraperitoneal injections are not ideal is given in a study where the best route of administration was determined for cancer biotherapy. All authors have reviewed the final version of the manuscript and approve it for publication. and JavaScript. & Gratwohl, A. TSG-6 might be a major anti-inflammatory mechanism of MSCs, since not only in colitis model, the therapeutic effect of TSG-6 remotely secreted by MSCs were also identified in many other disease model46,48,49,50. While the DiR labeled cells showed very different cell distribution. Kalchenko, V. et al. National Cancer Institute. The number of therapeutic cells that can migrate and colonize at the injury site is a decisive prerequisite for the success of cytotherapy. Google Scholar. Article Ulcerative colitis (UC) and Crohns disease (CD) are most common conditions in IBD. The accessibility of the therapeutic cells to their expectable target site is strongly dependent on the route of administration used. Therapeutic strategies should be based on a sound and thorough understanding of the disease mechanisms, if possible, however the causes of IBD are still unclear6. [5] In 1967, the production of amnesia was studied through an injection of physostigmine. A disadvantage of using intraperitoneal injections is that they can have a large variability in effectiveness and misinjection. Google Scholar. Of the time points we tested (6, 16, 24 h) our results showed that a 16-h post-intraperitoneal injection image scan provided the best contrast in mice regardless of age or disease burden. Accessibility There are few accounts of the use of intraperitoneal injections prior to 1970. This shows an important limitation to the use of IP therapy. The supernatants were diluted 1:30 with assay buffer consisting in 50mM phosphate buffer pH 6.0 with 0.167mg/mL o-dianisidine (Sigma) and 0.0005% H2O2.The colorimetric reaction was measured at 450nm between 1 and 3min in a spectrophotometer (Varioskan Flash Multimode Reader, Waltham, MA). Indeed, Mariana et al. To determined cytokine in colon mucosa, protein extracts were isolated by homogenization of colonic segments (50mg tissue/mL) in 50mmol/L Tris-HCl, pH 7.4, with 0.5mmol/L dithiothreitol and 10 g/mL of a cocktail of proteinase inhibitors (Sigma). [4] In 1964, a study delivered chemical agents such as acetic acid, bradykinin, and kaolin to mice intraperitoneally in order to study a "squirming" response. In IV group, most of MSCs were trapped in the lung, liver and spleen, while the cell that immigrated to the colon was not too much. World journal of gastroenterology 20, 1822818239, doi: 10.3748/wjg.v20.i48.18228 (2014). CAS Gastroenterology 132, 944954, doi: 10.1053/j.gastro.2006.12.029 (2007). Infiltration (I): 0, no infiltrate; 1, infiltrate around crypt basis; 2, infiltrate reaching to lamina muscularis mucosa layer; 3, extensive infiltration reaching the muscularis mucosa with abundant edema; 4, infiltration of the submucosa layer. In order to effectively administer drugs through IP injections, the stomach of the animal is exposed, and the injection is given in the lower abdomen. In another Phase I clinical trial, patients with ovarian cancer were injected intraperitoneally with dl1520 in order to study the effects of a replication-competent/-selective virus. In our study, we tracked the GFP+ MSCs colon colonization, but our results also showed the short stay of MSCs after injection. Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? The optimum route of administration is an importance clinical issue not only for chemical drug, but also for stem cytotherapy. Though IP injection showed benefits in many human and animal studies, even recommend by National Cancer Institute (NCI) for advanced ovarian chemotherapy, it is more often applied to animals than to humans for the time being57. They found that PCs can directly migrated from peritoneal cavity and some of them were found at intestinal villi. n=68 mice/group; n=3 for the control and naive (health mice) group. Li, C. Y. et al. 1A) showed the time point of DSS given and MSCs therapy. Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. IP, intraperitoneal injection; IV, intravenous injection; AI anal injection; n = 6-8 for treatment group, n = 3 for DSS control. Gastroenterology 140, 17851794, doi: 10.1053/j.gastro.2011.01.055 (2011). However, no significant TSG-6 difference was found between AI and IV groups. Surg Clin North Am 87, 727741, doi: 10.1016/j.suc.2007.03.004 (2007). & Dennis, J. E. MSCs: Delivery Routes and Engraftment, Cell-Targeting Strategies and Immune Modulation. A study was conducted to determine the best route of administration to transplant mesenchymal stem cells for colitis. After 5 days of DSS contained water, all the subject mice showed strong positive test of fecal occult blood. All reactions were performed in triplicated. Department of Digestive Diseases, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xian, 710032, China, Min Wang,Cong Liang,Hao Hu,Lin Zhou,Bing Xu,Xin Wang,Ying Han,Yongzhan Nie,Jie Liang&Kaichun Wu, Department of Gastroenterology, Xian Childrens Hospital, 710006, China, Department of Respiratory and Gastroenterology, Second Peoples Hospital, Xian, 710005, China, Department of Gastroenterology, PLA No.5 Hospital, Yinchuan, 750004, China, You can also search for this author in Then sections were washed and blocked with normal nonimmunone goat serum for 30min. 2014 Apr 26;(4):CD005284. Injections Intraperitoneal (IP) "Mouse" Once animal is restrained turn over so abdomen is exposed. In patients with peritoneal surface malignancies, perioperative IP chemotherapy has been used with good results. Myd88-dependent positioning of Ptgs2-expressing stromal cells maintains colonic epithelial proliferation during injury. intraarticular and intraperitoneal use of opioids, pain management in the emergency room and in the intensive care unit, pain management issues during pregnancy, geriatric pain, and hospice care and end-of-life issues. physiology - Why are some human injections intraperitoneal? - Biology evaluated the therapeutic efficacy of different MSCs sources and delivery routes in experimental emphysema37. anesthesia). In 1996, intraperitoneal (IP) administration of cisplatin plus intravenous (IV) cyclophosphamide proved superior to both drugs given intravenously at the same doses-which, at the time, was the standard treatment in the United States. The expression level of TNF-, the most important pro-inflammatory cytokine, was markedly decreased in IP group, compared with AI (P=0.0174), IV (P=0.0019) and control group (P<0.001), respectively. We believe with the increasing interests for IP injection in the field of cytotherapy, more innovation and creative ideas for reducing IP complications will come up. Note where each injection is made and subsequent injections should be made in slightly different spot than the previous injection. These findings may benefit clinic practice. Half of the volume of your D-luciferin substrate can be injected on one side of the animal and the other half of the volume on the opposite side. PMC PDF An improved mouse model of sepsis based on intraperitoneal injections A . [14] The symptoms of this included impairment of cognitive and psychomotor abilities. [10] The results depicted that ketobemidone consumption was significantly lower when patients controlled anesthetic through IP. Intramuscular. Journal of molecular neuroscience: MN 44, 178185, doi: 10.1007/s12031-010-9446-6 (2011). and H.H. & Dittel, B. N. Isolation of mouse peritoneal cavity cells. Choi, H., Lee, R. H., Bazhanov, N., Oh, J. Y. This study included unilateral SSNHL patients who were hospitalized in our department between . [2], There has been some debate on whether intraperitoneal injections are the best route of administration for experimental animal studies. In this study, three different MSCs delivery routes, intraperitoneal injection (IP), intravenous injection (IV) and anal injection (AI) were compared on DSS-induced colitis mouse model. RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio Rad, Hercules, CA). 5C). Liotta, F. et al. Most importantly, IP was found to have highest serum TSG-6 at both 48h and 72h and showed statistical significance when comparing with AI and DSS control at 72h. These findings, to some extent, may help to understand why IP is better than AI and IV. Ballinger AE, Palmer SC, Wiggins KJ, Craig JC, Johnson DW, Cross NB, Strippoli GF. [2] Killing the rodent through an intraperitoneal route was originally recommended over other routes such as inhalants because it was thought to be more efficient and ethical. Shariati M, Zhang H, Van de Sande L, Descamps B, Vanhove C, Willaert W, Ceelen W, De Smedt SC, Remaut K. Pharm Res. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Schrepfer, S. et al. Currently, therapy still largely relies on empirical and often implemented in a stepwise fashion: progressing through 5-aminosalicylate compounds, corticosteroids, immunomodulatory drugs and finally anti-TNF drugs7. Course of Crohns disease after allogeneic marrow transplantation. PubMed Intraperitoneal (IP) Injection in Rats and Mice SOP Page 4 of 6 Mouse Rat 6. humans have been associated with clinical pain, inflammatory lesions, and abscess formation in . However, human insulin . PubMed Quantitative data are presented as the meanSD. Researchers has tried many creative ways to minimize these risks. CAS Taede et al. Stem cells and development 22, 181192, doi: 10.1089/scd.2012.0373 (2013). Scientific Reports (Sci Rep) An improved mouse model of sepsis based on intraperitoneal injections of the enriched culture of cecum slurry . These non-target entrapments made IV route less cells immigration and colonization and may be a hindrance for MSCs to fully display its therapeutic effects. Sonazoid was utilized to aid with contrast-enhanced ultrasonography by enhancing the peritoneal and pleural cavities. Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. Intraperitoneal Injection of Oncolytic Viruses H101 for Patients With Journal of biomedical optics 11, 050507, doi: 10.1117/1.2364903 (2006). The levels of immunomodulatory cytokines were compared by real time-PCR and ELISA. This case presents the question of how THC is absorbed in the peritoneal cavity. In order to compare the difference of MSCs colonization among the three delivery routes, a nontoxic NIR tracer DiR was introduced to label MSCs. Intraperitoneal (IP) Injection (Injection of Sodium Pentobarbital . Intraperitoneal injections as an alternative method for micro-CT designed the research. Lopez-Cubero, S. O., Sullivan, K. M. & McDonald, G. B. Thus, we have succeeded to produce patient-like tumor colonization of the peritoneal cavity and hepatic metastasis with the human EAC cell line OE19 by intraperitoneal injection in SCID mice. In quantification study, the intensity of engrafted cells in IP and AI groups were significant higher than the IV (P=0.004, P=0.0012; Fig. Increased initial cell dose ensures an increased number of cells that reach to the injury site. . Opioids can produce peripheral analgesic effects by activation of opioid receptors on sensory nerves. Total RNA was extracted with the RNAeasy Plus kit (QIAGEN). However, whether the lymphocytes actually . This case is a good example of how IP injections can be used to deliver a drug that can help to treat or cure a medical diagnosis over the use of other routes of delivery.
How To Reset Kenmore Series 500 Washer,
Characteristics Of Mathematical Language,
Liveaboard Boat For Rent Brighton,
Articles I